Inhibition of IL-17 and IL-23 in Human Keratinocytes by the A₃ Adenosine Receptor Agonist Piclidenoson

Figure 1

A₃AR overexpression in a skin lesion and PBMCs from psoriasis patients. (a) A₃AR expression level was evaluated in formalin-fixed paraffin-embedded representative skin lesion from psoriasis patient. A₃AR mRNA in the psoriatic skin lesion was compared to adjacent healthy skin tissue using RT-PCR. A₃AR was highly expressed in the skin lesion compared to the healthy tissue. (b) A₃AR protein expression level was analyzed using WB analysis in PBMCs from psoriasis patients () compared with healthy subjects (). A significant () A₃AR upregulation is noted. (c) A₃AR mRNA expression level was tested in PBMCs from psoriasis patients compared to healthy subjects using real-time PCR.

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