Liver HLA-E Expression Is Associated with Severity of Liver Disease in Chronic Hepatitis C

Figure 1

(a) HLA-E positivity in hepatocytes (red arrow) and Kupffer cells (blue arrow). (b) To analyze the identity of intrahepatic HLA-E-positive cells, liver samples were double-labeled for HLA-E and CD68. All sections were immunostained with the indirect immunoperoxidase method and counterstained with hematoxylin. In double-labeling experiments, we incubated the sections with CD68-specific antibodies after performing the HLA-E-specific immunoperoxidase reaction. A: HLA-E-specific immunoperoxidase reaction (yellow); B: anti-CD68 (red) to confirm that these positive cells were Kupffer cells.

(a)

(b)