Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice

Figure 2

SCIT with Pru p 3, but not with R/A Pru p 3, suppressed development of anaphylactic reaction. (a) Immunization schedule. BALB/c mice were sensitized with Pru p 3 plus lipopolysaccharide (LPS) intranasally three times in the first week and subsequently at one-week interval four times with a total of 7 doses. One week after the final sensitization, mice received SCIT with 10 or 100 μg of Pru p 3 or R/A Pru p 3 at one-week interval in a total of eight times. One week after the final SCIT, the mice received intraperitoneal challenge with Pru p 3. (b) Left: dots represent drop of body core temperature after the challenge with Pru p 3 (x-axis). Right: clinical score according to a scoring system: 0: no symptoms; 1: scratching and rubbing around the nose and head; 2: puffiness around the eyes and mouth, diarrhoea, “pilar erecti,” reduced activity, and/or decreased activity with increased respiratory rate; 3: wheezing, laboured respiration, and cyanosis around the mouth and the tail; 4: no activity after prodding or tremor and convulsion; and 5: death. Symbols represent individual mice in each group.

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