Immune Response and Partial Protection against Heterologous Foot-and-Mouth Disease Virus Induced by Dendrimer Peptides in Cattle

Figure 2

Antibody detection by ELISA in vaccinated cattle. Animals were immunized on days 0 and 18 (arrow) with B₂T or B₄T vaccine. (a) Kinetics of anti-peptide serum antibodies. Bars represent the mean IgG titres from bovines in each group (gray, B₂T; black, B₄T) throughout the experiment (error marks, SD). (b, c) Kinetics of total IgG and IgG1 anti-FMDV O1/Campos/Bra/58 serum antibodies. Titres were calculated as log₁₀ of the last reciprocal dilution above cut-off. Data points represent the IgG titre (b) or IgG1 titre (c) from each animal represented by different shapes (right legend) throughout the experiment. (d) FMDV-specific mucosal IgG1 and IgA responses. Nasal swabs were collected at 22 dpv. Each point represents the nasal IgG1 anti-FMDV antibody titres (log₁₀) (black) or IgA (gray) anti-FMDV O1/Campos/Bra/58 antibody level of each animal. The cut-off was established as the mean value of mock-vaccinated animals plus twice the SD value (dotted line).

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