Lymph Node-Targeting Nanovaccine through Antigen-CpG Self-Assembly Potentiates Cytotoxic T Cell Activation

Figure 4

The clearance kinetics in situ and accumulation kinetics of different vaccine formulations in lymph nodes. (a) Mice were immunized with Cy5-OVA or Cy5-OVA-CpG NPs. Then, mice were subjected to fluorescence imaging of the injection site different time points, and the corresponding fluorescence intensity was quantified (right). (b) Quantitative sum fluorescence intensity of removed proximal lymph nodes at various time points. The relative fluorescence intensity was normalized to the peak fluorescence intensity in the Cy5-OVA group. The corresponding area under the curve represents the relative accumulated fluorescence intensity and was normalized to the accumulated fluorescence intensity in the Cy5-OVA group. (c) Representative fluorescence images of mice (lymph nodes are circled with a yellow dotted line) and harvested lymph nodes at 8 h (first line: distal LNs; second line: proximal LNs). The bars represent sd ().

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