Research Article
A Quantitative Method for Detecting Ara h 2 by Generation and Utilization of Monoclonal Antibodies
Table 1
Positive clones selected after fusion and the first subcloning.
| | Clones selection after fusion | Clones selection after the first subcloning | | Clones | OD at 450 nm | Clones | OD at 450 nm |
| | Neg. ctrl | 0.051 | Neg. ctrl | 0.050 | | Pos. ctrl | 3.46 | Pos. ctrl | 2.360 | | 1-E4 | 3.042 | 1-1E4 | 2.26 | | 4-B10 | 3.051 | 4-1B10 | 1.796 | | 5-E2 | 3.867 | 5-1E2 | 2.233 | | 5-E12 | 3.654 | 5-1E12 | 2.133 | | 6-G9 | 3.676 | 6-1G9 | 2.4 | | 8-D10 | 3.642 | 8-1D10 | 0.057 | | 8-F8 | 3.846 | 8-1F8 | 0.055 | | 8-H11 | 3.533 | 8-1H11 | 0.07 |
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Neg. ctrl: negative control, serum from nonimmunized mouse. Pos. ctrl: positive control, serum from immunized mouse.
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