CD47 Blockade Inhibits Tumor Progression through Promoting Phagocytosis of Tumor Cells by M2 Polarized Macrophages in Endometrial Cancer

Figure 4

CD47 knockdown increases phagocytosis of EC cells by macrophages. (a, c) Successful knockdown of CD47 in EC cell lines (Ishikawa cell line and KLE cell line) measured by flow cytometry. (b, d) Representative images of the phagocytosis assay in which CD47-knockdown EC cells (Ishikawa or KLE cells) or control EC cells were cocultured with NSG mouse BMDMs. Percentages of CFSE⁺ F4/80⁺ macrophages in total macrophages were indicated beside the gated population. Data are shown as the mean ± SEM (ns, not significant; and ).

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