CD47 Blockade Inhibits Tumor Progression through Promoting Phagocytosis of Tumor Cells by M2 Polarized Macrophages in Endometrial Cancer

Figure 5

CD47 blockade increases phagocytosis of EC cells by M2 macrophages. (a-b) Representative images of the phagocytosis assay in which Ishikawa cells were cocultured with M1 or M2 NSG mouse BMDMs in the presence of anti-CD47 antibody or control IgG antibody. The white arrows point to the macrophages that phagocytosed Ishikawa cells. Scale bars, 20 μm. (c) Flow cytometry results of phagocytosis assays. Percentages of CFSE⁺ F4/80⁺ macrophages in total macrophages were indicated beside the gated population. Data were shown as the mean ± SEM (ns, not significant; ).

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