Research Article
Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines
Figure 4
No detection of β2m cDNA (a) and the B2M promoter-to-intron 1 region (b) in both sHCC29 and sHCC63 cells by RT-PCR and genomic PCR, respectively, as compared to sHCC74 and SAR-HCV cells. Schema illustrates the putative deleted region of the B2M gene in the two HLA class I-negative sHCC29 and sHCC63 cell lines (c). MFI: mean fluorescence intensity. See the text for detailed explanation.
| (a) |
| (b) |
| (c) |