Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines

Figure 5

Differential expression of β₂m, LMP2, LMP7, TAP1, and TAP2 mRNA in the three newly established sHCC cell lines cultured in the presence or absence of IFN-γ (300 U/mL, 48 h). Upper panel: agarose gel stained with ethidium bromide shows the electrophoresis of end point RT-PCR products for the tested mRNAs of the three sHCC cell lines. Lower panel: quantification of RT-PCR results from three independent experiments where relative mRNA expression of each gene was normalized to glyceraldehydes 3-phosphate dehydrogenase (GAPDH) and expressed as mean fold-changes ± SD. LMP: low-molecular-weight protein; TAP: transporter associated with antigen processing.