Research Article
Total HLA Class I Antigen Loss with the Downregulation of Antigen-Processing Machinery Components in Two Newly Established Sarcomatoid Hepatocellular Carcinoma Cell Lines
Figure 6
Two-color cytofluorometric analysis of the sHCC29, sHCC63, sHCC74, and SAR-HCV cell lines using mAbs to surface markers CD24 and CD44 conjugated with allophycoyanin (APC) and fluorescein isothioyanate (FITC), respectively (a). Immunohistochemical staining patterns of the tumor lesions from which the sHCC29, sHCC63, and sHCC74 cell lines were established using mAbs to HLA-ABC (with mAb W6/32), heavy chain (mAb HC-10), and β2m (mAb L368). Isotype-matched Ig serves as the negative control (b).
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