CD11c-Specific Deletion Reveals CREB as a Critical Regulator of DC Function during the Germinal Center Response

Figure 1

Steady-state DC homeostasis is not affected by CREB deficiency: (a) percentages of CD11c⁺ cells in different lymphoid organs of indicated mice determined by flow cytometry; (b–d) activation marker expression in CD11c⁺ cells from WT and CKO mice ( each; mean fluorescence intensity was calculated with error bar representing SEM); (e) percentages of cDCs of indicated mice determined by flow cytometry; (f) percentages of pDCs of indicated mice determined by flow cytometry; (g) percentages of CD11c-expressing cells after the in vitro differentiation of BMDCs with GM-CSF and IL-4 for 7 days; (h) activation markers in CD11c-expressing cells after the in vitro differentiation of BMDCs with GM-CSF and IL-4 for 7 days (mean fluorescence intensity was calculated with error bars representing SEM). (i) Differentiated BMDCs of WT or CKO animals were either left untreated or were pulsed with OVA peptide (1 μM) for 2 h and extensively washed with PBS. BMDCs were cocultured with cell proliferation dye labeled T Cells of OVA-transgenic OT-II mice. FACS analysis of T cells was performed after 3 days. The number represents percentage of proliferated cells in each quadrant (). (j) Representative density plot of (i). For (a, e, f, and g), each dot represents an individual mouse, and horizontal bars indicate SEM.

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