Impaired Gal-9 Dysregulates the PBMC-Induced Th1/Th2 Imbalance in Abortion-Prone Matings

Figure 4

Effect of galectin-9 blockade and recombinant galectin-9 treatment on in vitro Th1/Th2 cytokine expression from coculture supernatants of abortion matings. IL-2 (a), IFN-γ (b), and IL-4 (c) expression in coculture supernatants of trophoblastic cells with PBMCs isolated from abortion-prone matings, with or without anti-galectin-9-Ab administration. IL-2 (d), IFN-γ (e), and IL-4 (f) expression in coculture containing trophoblastic cells and PBMCs, with or without recombinant Gal-9 administration. All cytokines were collected and detected at d 1, d 3, and d 6 by ELISA. All results are presented as means ± SEM of at least three independent experiments (). ; .

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