High-Titre Neutralizing Antibodies to H1N1 Influenza Virus after Mouse Immunization with Yeast Expressed H1 Antigen: A Promising Influenza Vaccine Candidate

Figure 5

ELISA of the glycosylated and deglycosylated H1N1pdm09 recombinant protein using FI6 antibody. The H1 protein was deglycosylated with Endo H in native condition (pH 7.0). Two concentrations of the H1 protein were tested. Efficient HA-mediated haemagglutination was observed at high H1 concentration (data not shown). This is consistent with previous data, which suggested that only high HA oligomers are able to bind to multiple red blood cells to create the latticed structures that are measured in the haemagglutination assay [10].