Crotalus durissus ruruima Snake Venom and a Phospholipase A₂ Isolated from This Venom Elicit Macrophages to Form Lipid Droplets and Synthesize Inflammatory Lipid Mediators

Figure 7

Both CdrV- and CBr-induced LDs are involved in the synthesis of PGE₂. Elicited macrophages incubated with RPMI (control) or CdrV (10 μg/mL) or CBr (6.5 μg/mL) for 3 h were labeled for LDs (Nile Red) and for PGE₂ (anti-PGE₂ antibody). The merged image shows colocalization of PGE₂ to LDs. The pictures are representative of three independent experiments with 3 to 5 animals.