Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells

Figure 5

Expression of mRNA for fibrogenic proteins and specific cell marker mRNA in early B-cell depletion. RQ values are shown from the early B-cell depletion experiment; RAG2 ^-/-γc^-/- mice injected with huPBMCs with Rituximab induced B-cell depletion (indicated as +) and RAG2 ^-/-γc^-/- mice injected with huPBMCs without this depletion (indicated as -), for the most important observations, concerning human and murine TGF-β, human IFN-γ, and different human cell types, including FoxP3 (Tregs), GATA3 (Th2 cells), and Tbet (Th1 cells). All other results were either not significant (CD4, CD8, murine CD68, murine CTGF, murine BMP4), had cycle threshold (Ct) (CD20 and human CD68) or were not detectable at all (human factors FGF2, PDGF, EGF, BMP4 and CTGF).

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