Increased TLR4 Expression Aggravates Sepsis by Promoting IFN-γ Expression in CD38^−/− Mice

Figure 5

The elevated expression of mRNA of proinflammatory cytokines and increased expression of IFN-γ in serum induced by LPS challenge in CD38^−/− mice were rescued in the CD38^−/−TLR4^mut mice. Expression of TLR4 in the kidney was measured 2 hours after LPS injection. (a) A significant increased expression of mRNA level of TLR4 in the CD38^−/− mice. (b) A significant decreased expression of mRNA level of TLR4 in the CD38^−/−TLR4^mut mice. (c) Expression of protein level of TLR4 is increased significantly in the kidney of the CD38^−/− mice and decreased in the kidney of the CD38^−/−TLR4^mut mice. Changes in proinflammatory cytokine expression in the CD38^−/−TLR4^mut mice compared with the CD38^−/− mice two hours after LPS injection. (d–g) Expression of IFN-γ, TNF-α, IL-1β, and IL-6 mRNA in the kidney. (h–k) Expression of IFN-γ, TNF-α, IL-1β, and IL-6 in serum. Data are presented as deviation (SD). Statistical significance was determined by the unpaired -test. , , .

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