Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro

Figure 7

Modulation of TLR-negative regulator expressions by TCF of LABs in HepG2 cells not treated with free fatty acid (normal cells). HepG2 cells were prestimulated with total cellular fluid of LABs and poststimulated with LPS for 3 h and 12 h. The expressions of A20, SIGIRR, Tollip, and IRAK-M1 were determined by RT-PCR. HepG2 cell treated with LPS was used as control. Different superscript letters indicate significant differences at the 0.05 level.