MHC Class I Molecules Exacerbate Viral Infection by Disrupting Type I Interferon Signaling

Figure 2

MHC I inhibited IFN downstream signaling. (a) Western blot analysis of STAT1 phosphorylation in WT and MHC I^-/- macrophages post VSV infection. (b) Relative density of the blot in (a). (c) Representative ISG expression in WT and MHC I^-/- macrophages post VSV infection. (d) Western blot analysis of STAT1 activation in WT and MHC I^-/- macrophages treated with IFN-β for the indicated times. (e) Relative density of the blot in (d). (f) Phosphorylation of MHC I after coimmunoprecipitation with anti-H2Kb antibody visualized by immunoblot with anti-p-Tyr. (g) Relative density of the blot in (f). Data are the of at least three independent experiments. Two-way ANOVA was adopted for statistical analysis in (b), (e), and (g). Unpaired Student’s -test was adopted for statistical analysis in (c). .

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