Intranasal Application of Budesonide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Suppressing Nucleotide-Binding Oligomerization Domain-Like Receptor Family, Pyrin Domain-Containing 3 Inflammasome Activation in Mice

Figure 7

Budesonide suppressed NLRP3 inflammasome activation in macrophages in vitro. RAW 264.7 cells were treated with LPS (100 ng/mL) + ATP (5 mM) with or without budesonide. The protein expression levels of NLRP3 (a, b) and caspase-1 p20 (a, c) were detected using western blotting. The concentrations of IL-1β in the supernatant were detected using ELISA (d). Data were expressed as deviations (). compared with the control group, compared with the LPS + ATP group, and compared with the LPS + ATP group.

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