Sildenafil Citrate Influences Production of TNF-α in Healthy Men Lymphocytes

Figure 2

Effect of sildenafil citrate on the percentage of TNF-α- and IFN-γ-positive T cell subsets after PHA stimulation of PBMC cultures. The error bars represent the standard deviation. Abbreviations: CD3, CD4, and CD8—PBMC, CD3 SC 400 ng/ml, CD4 SC 400 ng/ml, and CD8 SC 400 ng/ml—PBMC+SC, PHA: phytohemagglutinin at a concentration of 2 μg/ml, PBMC: peripheral blood mononuclear cells, SC: sildenafil citrate (); please refer to the supplementary information for gating strategy.