Efficient Therapeutic Function and Mechanisms of Human Polyclonal CD8⁺CD103⁺Foxp3⁺ Regulatory T Cells on Collagen-Induced Arthritis in Mice

Figure 5

The mechanisms of human CD8⁺Treg-controlled CIA mice in vivo. (a) The influence of human CD8⁺ Tregs on splenic CD4⁺ T cell subtypes in CIA mice. Splenic cells were isolated, and the CD4⁺ T cell subtypes were determined after 72 hours of transfer. The levels of Foxp3, IL-17A, and IFN-γ in mouse CD4⁺T cells were evaluated by FACS. The right graph shows the representative FACS data, and the left graph indicates the statistical results. (b) mRNA expression of cytokines and related factors in CIA mouse feet. CIA mice were sacrificed 72 hours after human CD8⁺Treg transfusion; then, their paws were minced and digested, and the cells were collected. Subsequently, mRNA was measured by real-time PCR. The results are expressed as the of at least three independent experiments; , , and , compared with the control group by unpaired -tests.

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