Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis

<div>Splenic neutrophils help in the immune response of MZ B cells. (a) Neutrophils were depleted by injecting 1A8 monoclonal antibody, and the depletion efficiency was measured using flow cytometry analysis. After intravenous immunization with T cell–independent antigen NP-Ficoll, (b) serum levels of NP-specific IgG3 and IgM in the neutrophil-deficient mice were determined using ELISA. (c) qRT-PCRs reveal the mRNA levels of AID (<i>AICDA</i>) and BLIMP-1 (<i>PRDM1</i>) for the isolated MZ B cells from the immunized neutrophil-sufficient or -deficient mice. (d) Ly6G-positive neutrophils are shown to associate with the plasma cells (CD138+) in the bone marrow. (e) After neutrophil depletion, the apoptosis in the bone marrow plasma cells were assessed using Annexin V staining. 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Journal of Immunology Research

fig2

Figure 2

Figure 2: Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis 