Neutrophil-Derived MRP14 Supports Plasma Cell Commitment and Protects Myeloma Cells from Apoptosis

Figure 3

MRP14 expression in the splenic neutrophils and correlation with MZ B cell. Expression of MRP14 mRNA in (a) immune cells and (b) various mouse tissues. The data are from BioGPS and are presented as raw expression values. (c) Anti-MARCO stains the MZ macrophages, and MRP14 staining exhibits in the spleen peri-MZ region. Costaining with Ly6G antibody shows the highly concentrated MRP14 on the splenic neutrophils. Scale bars represent 100 μm. (d) Flow cytometry histograms show that substantial amounts of MRP14 protein are present on the surface of splenic neutrophils and MZ B cells. (e) Anti-collagen type IV stains vessels and sinus in the bone marrow. Costaining shows the correlation between MRP14 staining and Ly6G+ neutrophils. Scale bars represent 100 μm. (f) Flow cytometry histograms show the surface presence of MRP14 protein on the neutrophils and the plasma cells in the bone marrow. Data shown represent means at least six mice from three independent experiments.

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