Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro

Figure 2

Heat shock-conditioned OECCL lysates induce maturation and activation-associated marker expression on monocyte-derived DCs. The surface expression of MHC I, MHC II, CD80, CD83, CD86, CD40, and CCR7 was evaluated by flow cytometry on CD11c⁺ IL-4/GM-CSF-activated monocytes unstimulated (AM) or stimulated for 24 hours with LPS (1 μg/mL) and the heat shock-conditioned lysates TRIMEL, MOVL1, or MOVL2 (100 μg/mL). The results were obtained from multiple independent experiments and are showed as fold change relative to unstimulated AM. Statistics compare treatments to AM; , , and ^#.