Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro

Figure 5

Activation of autologous T cells by monocyte-derived DCs maturated with heat shock-conditioned OEC lysates. Sorted CD4⁺ (a) and CD8⁺ (b) T cells were kept alone (LT alone) or cocultured for 14 days with autologous IL-4/GM-CSF-activated monocytes unstimulated (AM) or maturated for 24 hours with 100 μg/mL of heat shock-conditioned lysates TRIMEL, Hey, MOVL1, or MOVL2 (TRIMEL-DC, Hey-DC, MOVL1-DC, and MOVL2-DC, respectively). The expression levels of CD25 and CXCR3 on CD4⁺ (a) or CD8⁺ (b) T cells were analyzed by flow cytometry and showed as mean fluorescence intensities (MFI). The results were obtained from multiple independent experiments. Statistics compare treatments to AM; ; , and ^#.

(a)

(b)