Dendritic Cells Loaded with Heat Shock-Conditioned Ovarian Epithelial Carcinoma Cell Lysates Elicit T Cell-Dependent Antitumor Immune Responses In Vitro

Figure 6

Monocyte-derived DCs maturated with heat shock-conditioned OEC lysates induce antitumor effector responses in autologous CD4⁺ and CD8⁺ T cells. Sorted CD4⁺ (a) and CD8⁺ (b) T cells were kept alone (LT alone) or cocultured for 14 days with autologous IL-4/GM-CSF-activated monocytes unstimulated (AM) or maturated for 24 hours with 100 μg/mL of heat shock-conditioned lysates TRIMEL, Hey, MOVL1, or MOVL2 (TRIMEL-DC, Hey-DC, MOVL1-DC, and MOVL2-DC, respectively). After coculture with the different DCs, CD4⁺ (a) and CD8⁺ (b) T cells were sorted and challenged with different target tumor cells (Hey, CAOV3, Mel1, and K562). (a) IFN-γ secretion was evaluated by ELISPOT assays. (b) The lysis of the target cells was evaluated by ⁵¹Cr release assays. The results were obtained from multiple independent experiments. Statistics compare treatments to AM; and ^#.

(a)

(b)