miR-98 Modulates Cytokine Production from Human PBMCs in Systemic Lupus Erythematosus by Targeting IL-6 mRNA

Figure 5

STAT3 regulates IL-6-mediated miR-98 functions in SLE PBMCs. PBMCs from SLE patients were transfected with miR-98 inhibitor or NC, IL-6 overexpression vector or empty vector, and/or S3I-201; cell growth was analyzed by MTT assay (a); the expression of TNF-α, IL-8, IL-1β, and IL-10 in cells was detected by qRT-PCR (b); the levels of TNF-α, IL-8, IL-1β, and IL-10 in a medium was tested by ELISA (c, d). Data are means of three separated ; .

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