miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob

Figure 1

miR-223-3p was downregulated in OVA-induced DCs. (a) Mouse immature DCs were treated with 100 μg/ml OVA for 24 h and then stained with specific Abs against MHC-II, CD80, and CD86 for flow cytometry analysis. (b) The bar chart indicated the MFI of the DCs in each group. -test, , compared to the control group. (c) miR-223-3p expression in OVA-treated immature DCs. Immature DCs were incubated with OVA (100 μg/ml) for 24 h. Cells were collected at the indicated time points, and miR-223-3p expression was determined by RT-PCR. Data were shown as of three independent experiments. Post hoc Tukey’s HSD test, , , compared to the 0 min control.

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