miR-223-3p Inhibits Antigen Endocytosis and Presentation and Promotes the Tolerogenic Potential of Dendritic Cells through Targeting Mannose Receptor Signaling and Rhob

Figure 3

miR-223-3p suppressed surface molecule MHC-II expression on the OVA-induced BMDCs. Mouse immature DCs were transfected with the miR-223-3p inhibitor (a), inhibitor control, miR-223-3p mimic (b), or mimic control at a final concentration of 50 nM. After 24 h, DCs were stimulated with 100 μg/ml OVA for 24 h and then stained with specific Abs against MHC-II, CD80, CD86, and CD40 and then analyzed by flow cytometry. The bar chart indicated the MFI in the gate of CD11c+ cells in each group. Similar results were obtained in three independent experiments. Data were shown as . -test, , .