M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin

Figure 4

Cell migration and invasion of AGS cells. (a) AGS cells were plated in 0.8 μm pore size Transwell and incubated for 24 h. After 24 h incubation, AGS cells were treated with 10 μg/ml of 5FU in FBS free media, and the inserts were added in wells containing polarized THP-1-M2 macrophages or fresh media and incubated for further 24 h. After 24 h, nonmigrating cells on the upper side of the membrane were removed and migrated cells on the lower side of the membrane were fixed with ice-cold methanol. Fixed membranes were stained with 0.5% crystal violet. (b) Western blot analysis of MMP-2 and MMP-9 proteins. (c) Quantification of western blot bands. Statistically significant difference was set at .

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