Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo

Figure 3

Characterization of granulocyte-macrophage colony-stimulating factor (GM-CSF) generated bone marrow-derived dendritic cells (BMDCs) by flow cytometry analysis. The BMDCs were gated first to exclude debris (left upper panel) and then to exclude nonviable cells (right upper panel). The expression of CD11c is illustrated by a histogram gated on a CD11c-positive subset (left middle panel). Moticam 10000 camera, attached to an inversion light microscope (20x magnification), was used to take an image of generated BMDCs (right middle panel). The expression of CD80, CD86, and MHC class II (I-A/I-E) molecules on surface-stained CD11c+ BMDCs (filled histogram) is illustrated with unstained cells (dotted line) by overlaid histograms (bottom panel) with median fluorescence intensity (MFI) values depicted in the boxes. Results are representative of three independent experiments.