Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo

<div>Internalization of norovirus GII.4 virus-like particles (VLPs) and rotavirus VP6 nanotubes. Flow cytometry analysis of norovirus GII.4 VLPs (a) or VP6 nanotubes (b) uptaken by bone marrow-derived dendritic cells (BMDCs) after 22 h incubation with 100 <i>μ</i>g/ml of proteins. The intracellular staining of unpulsed BMDCs (dotted line) or BMDC pulsed with the proteins (filled histogram) is shown by overlaid histograms with median fluorescence intensity (MFI) values depicted in the boxes. The percentual uptake of GII.4 VLPs and VP6 nanotubes is shown in the SSC-A versus GII.4-PE (a) or VP6-FITC (b) density plots of BMDCs gated on viable cells. The gates in the density plots are drawn according to the unpulsed BMDCs stained with similar procedures as the pulsed cells (data not shown).</div>

Journal of Immunology Research

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Figure 4: Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo 

Figure 4 | Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo 