Rotavirus VP6 Adjuvant Effect on Norovirus GII.4 Virus-Like Particle Uptake and Presentation by Bone Marrow-Derived Dendritic Cells In Vitro and In Vivo

Figure 7

The humoral immune responses generated by in vitro-pulsed BMDCs. Norovirus GII.4 virus-like particle- (VLP-) pulsed bone marrow-derived dendritic cells (BMDCs) were transferred intramuscularly two times to mice either alone (Gr II) or in combination with simultaneously (Gr III) or separately (Gr IV) VP6-pulsed BMDCs as described in Materials and Methods. Unpulsed BMDCs were used as negative control cells (Gr I). Mouse termination sera were tested individually (1 : 100 dilution) in GII.4- (left panel) and VP6- (right panel) specific IgG (a, b), IgG1 (c, d), and IgG2a (e, f) ELISAs. Group mean optical density (OD) values at 490 nm with standard error of the means (error bars) are shown. Statistically significant value.

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