miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT

Figure 2

miR-24-3p regulated KLF8, Snail, and E-cadherin expressions in lung adenocarcinoma cells, and KLF8 was a direct target of miR-24-3p. (a) qRT-PCR analysis of miR-24-3p expression in A549 and H1299 cells transfected with either miR-NC or miR-24-3p mimics. (b–d) mRNA expression of KLF8 (b), Snail (c), and E-cadherin (d) in A549 and H1299 cells transfected with either miR-NC or miR-24-3p mimics was detected by qRT-PCR. (e) Expression of KLF8, Snail, and E-cadherin in A549 and H1299 cells transfected with either miR-NC or miR-24-3p mimics was detected by Western blot. (f) miR-24-3p binding sites in 3-UTR of KLF8 were predicted by TargetScan. The seed region of miR-24-3p and the recognition site in the KLF8 3-UTR are shown in red. The sequences of the WT and MUT KLF8 3-UTR were used for the dual luciferase reporter construct. (g) The pMIR-REPORT vector and either KLF8-WT or KLF8-MUT plasmids were cotransfected with miR-24-3p or miR-NC mimics in H1299 cells, and the relative luciferase activity was detected. Data are . , .

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