High CXCR3 on Leukemic Cells Distinguishes IgHV^mut from IgHV^unmut in Chronic Lymphocytic Leukemia: Evidence from CD5^high and CD5^low Clones

Figure 5

Cooperative effect of CXCL11 and CXCL12 on the migratory ability of CLL cells. CLL cells (), with/without pretreatment with 200 ng/mL CXCL11, were added to the upper chamber of Transwell inserts. 200 ng/mL CXCL12 was added to the medium in the bottom chamber, and migrated cells were counted after 3 hours by flow cytometry. Migration index was calculated as the ratio of cells that transmigrated through the insert in the chemokine-treated cells vs untreated ones (CXCR11-/CXCR12-).