Research Article
Systematic Study of the Immune Components after Ischemic Stroke Using CyTOF Techniques
Figure 3
Cell surface markers significantly expressed in different immune cell subtypes from samples of sham, day 1, day 3, day 7, and day 14 poststroke in the brain, blood, spleen, and bone marrow. (a) The surface marker expression levels of specific immune cells according to their cell types in the ischemic brain. The heat map depicts the ArcSinh ratio of median values. The minimum value in each protein expression was used as a control. The cell surface markers include CD45 expression on MiMØs and MoMØs; B220 expression on B cells; CD4 expression on CD4+ T cells; CD8 expression on CD8+ T cells; CD44 expression on CD4 TEM and CD8 TEM cells; CD25 expression on Tregs; CD11c expression on cDCs; NK1.1 expression on NK cells; and Gr-1 expression on Gr-1+ cells. (b–d) Illustrative SPADE tree to present the identified immune cell types in the peripheral blood (b), spleen (c), and bone marrow (d) (left). The cell surface marker expression levels of specific immune cells’ on the corresponding immune cell types (right).
| (a) |
| (b) |
| (c) |
| (d) |