Elemental Diet Enriched with Amino Acids Alleviates Mucosal Inflammatory Response and Prevents Colonic Epithelial Barrier Dysfunction in Mice with DSS-Induced Chronic Colitis

Figure 4

An elemental diet enriched in amino acids inhibits the predominant Th1/Th17 responses in the colon of colitis-bearing mice. Flow cytometric analysis of CD3⁺CD4⁺ T (a), CD3⁺CD8⁺ T (b), CD4⁺ IFN-γ⁺ Th1 (c), and CD4⁺ IL17A⁺ Th17 cells (d) in the spleen, mesenteric lymph node (MLNs), and lamina propria mononuclear cells (LPMCs) from individual groups of mice were calculated. CD4⁺CD25⁺FoxP3⁺ Treg (e) frequency in CD4⁺ T cells was examined. Representative flow cytometric dot plots (left) and percentages of positive cells (right) are depicted ( per group). Data are representative images or expressed as the of each group from three separate experiments. , , .

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