Knockdown of YAP/TAZ Inhibits the Migration and Invasion of Fibroblast Synovial Cells in Rheumatoid Arthritis by Regulating Autophagy

Figure 2

Knockdown of YAP and TAZ in the lentiviral-transduced RA-FLS. (a, b) YAP and TAZ mRNA levels were evaluated from RA-FLS stably expressing shRNA specific for YAP (shYAP), TAZ (shTAZ), the corresponding vector control (shC), and null (Ctrl) using RT-qPCR and normalized against GAPDH. Data are the (). relative to shC by Student’s -test. (c) Knockout of YAP and TAZ was confirmed by western blot analysis in RA-FLS, and GAPDH served as a loading control. Representative blots are shown (). (d) Quantitative analysis of CTGF and CYR61 mRNA in stable YAP and TAZ knockdown RA-FLS by RT-qPCR and normalized against GAPDH. Data were shown as the (). relative to shC by Student’s -test.

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