Polypeptide Globular Adiponectin Ameliorates Hypoxia/Reoxygenation-Induced Cardiomyocyte Injury by Inhibiting Both Apoptosis and Necroptosis

Figure 4

The inhibition efficiency of gAd in hypoxia/reoxygenation-induced apoptosis and necroptosis compared with nec-1 and Z-VAD-FMK. (a, c, d) Western blotting shows that necrostatin-1 (nec-1, 100 mM), a specific blocker of necroptosis, inhibited the level of RIP1 and RIP3 and increased the level of cleaved caspase-3 (). (b) The expression of cleaved caspase-3 was increased in the nec-1 group after H/R (). (e, f) Z-VAD-FMK (50 mM), a cell-permeable pan-caspase inhibitor, suppresses the cleaved caspase-3 and increased the RIP1 and RIP3 expression (). (g, h) The expressions of RIP1 and RIP3were increased in the Z-VAD-FMK group after H/R (). ; .

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