Angiotensin II-Treated Cardiac Myocytes Regulate M1 Macrophage Polarization via Transferring Exosomal PVT1

Figure 2

Ang-II-stimulated HCM-derived exosomes promoted M1 macrophage polarization. Exosomes were collected from the culture medium of HCM treated with PBS or Ang-II. Then, the exosomes were incubated with THP-1 cells. (a) qRT-PCR was carried out to detect the expression of iNOS and TNF-α mRNAs in THP-1 cells. (b) The expression levels of Arg-1 and IL-10 mRNAs were also measured by qRT-PCR. (c) The percentages of CD86-positive and CD163-positive macrophage were analyzed using flow cytometry. (d) The expression of PVT1 in PBS-Exo and Ang-II-Exo was measured by qRT-PCR. (e) The expression of PVT1 in macrophage was measured by qRT-PCR. PBS-Exo: PBS-treated HCM-derived exosomes; Ang-II-Exo: Ang-II-stimulated HCM-derived exosomes. . compared to the PBS-Exo group and compared to the PBS-Exo group. Student’s -test was performed to analyze the difference between two independent groups, and the value of lower than 0.05 was considered as statistically significant difference.

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