Angiotensin II-Treated Cardiac Myocytes Regulate M1 Macrophage Polarization via Transferring Exosomal PVT1

Figure 3

Ang-II-treated HCM promoted M1 macrophage polarization through secreting exosomal PVT1. Lentivirus expressing shPVT1 or shCtrl was infected with Ang-II-stimulated HCM for 24 hours. (a) Exosomes were collected from the culture medium of HCM, and then, the expression of PVT1 in exosomes was measured by qRT-PCR. (b) These two types of exosomes were incubated with THP-1 cells. The expression of PVT1 in THP-1 cells was measured using qRT-PCR. (c) The gene expression of iNOS and TNF-α was detected using qRT-PCR. (d) qRT-PCR was carried out to examine the expression of Arg-1 and IL-10 mRNAs. (e) Flow cytometry was performed to ensure the percentage of M1 and M2 macrophage. Here, shCtrl-Exo was lentivirus-shCtrl-infected HCM-derived exosomes and shPVT1-Exo was lentivirus-shPVT1-infected HCM-derived exosomes. . and . Student’s -test was performed to analyze the difference between two independent groups, and the value of lower than 0.05 was considered as statistically significant difference.

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