Angiotensin II-Treated Cardiac Myocytes Regulate M1 Macrophage Polarization via Transferring Exosomal PVT1

Figure 4

PVT1 promoted IL-16 expression through acting as a miR-145-5p sponge. (a) Two different binding sequences between PVT1 and miR-145-5p were predicted using starBase. (b) The relationship between PVT1 and miR-145-5p was identified using luciferase reporter assay. . . (c) qRT-PCR analysis of PVT1 and miR-145-5p enrichment by Ago2 and IgG antibody in RIP experiments. . . (d) The binding sites between miR-145-5p and IL-16 3-UTR region were predicted using starBase. (e) Luciferase activity of THP-1 cotransfected with the reporter plasmid inserted WT or MUT IL-16 3-UTR sequences and miR-145-5p mimics, mimics NC, miR-145-5p inhibitor, or inhibitor NC. . . (f) The expression of IL-16 gene was measured by qRT-PCR. . compared with the Ctrl group and ^# compared with the PVT1 group. (g) The expression of IL-16 protein was determined using western blot. . compared with the Ctrl group and ^# compared with the PVT1 group. (h) qRT-PCR was performed to detect the expression of IL-16 gene. . compared with the PBS group, and ^# compared with the NC group. (i) Western blot was carried out to measure the expression of IL-16 protein. . compared with the PBS group, and ^# compared with the NC group. Student’s -test was performed to analyze the difference between two independent groups, and one-way ANOVA followed by Tukey’s post hoc test was carried out to ensure the difference among multiple groups. For all assays, the value of lower than 0.05 was considered as statistically significant difference.

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