Lipoprotein(a), an Opsonin, Enhances the Phagocytosis of Nontypeable Haemophilus influenzae by Macrophages

Figure 1

Purification of Lp(a). (a) Elution profile of Lp(a). A mixture of Lp(a) and LDL was run through a Q FF strong anion exchanger column and eluted with 300 mM NaCl, 20 mM Tris, and 1 mM EDTA (pH 7.4). The second peak corresponds to Lp(a). (b) SDS-PAGE and immunoblot analyses of Lp(a). SDS-PAGE gel stained with Coomassie blue (lane 1); an identical gel subjected to western blotting with either anti-apoB100 Ab (lane 2) or anti-apo(a) Ab (lane 3).

(a)

(b)