Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4<sup>+</sup> T Cells Isolated from Peripheral Blood Mononuclear Cells

<div>Treg cells after coculture of PBMCs with cADSC-EVs. (a) Flow cytometric detection of CD3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> cells (gated in red square). Control: PBMCs cultured without cADSC-EVs. (b) Comparison of the population of CD3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> cells after coculture of PBMCs with various concentrations (1 or 3 <i>μ</i>g/ml) of naive or primed cADSC-EVs. The ratio of Treg cells was not significantly different between naive and primed EVs at the same concentrations. The solid line indicates the average. <svg height="12.1645pt" id="M81" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -11.5275 54.2341 12.1645" width="54.2341pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,4.432,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.013,0,0,-0.013,9.395,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 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602Z"></path></g><g transform="matrix(.013,0,0,-0.013,50.025,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>,</span> between concentrations of 1 and 3 <i>μ</i>g/ml.</div>

Journal of Immunology Research

fig10

Figure 10

Figure 10: Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4<sup>+</sup> T Cells Isolated from Peripheral Blood Mononuclear Cells 

Figure 10 | Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells 