Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4<sup>+</sup> T Cells Isolated from Peripheral Blood Mononuclear Cells

<div>Differential expression of microRNAs in naive and primed cADSC-EVs. (a) Layout for Qiagen’s canine miScript miRNA PCR Array. (b) Heatmap of microRNA expression between naive and primed cADSC-EVs. Red an increase and green/black indicate a decrease in relative expression in primed cADSC-EVs (<svg height="8.55521pt" id="M11" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 28.424 8.55521" width="28.424pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M524 0V26C466 32 460 36 460 104V297C460 393 411 449 331 449C302 449 276 437 248 419C223 402 201 387 181 372V451C137 432 90 420 42 411V388C96 378 102 374 102 310V104C102 38 97 33 29 26V0H246V26C187 32 181 36 181 104V339C211 365 250 390 290 390C357 390 381 345 381 276V109C381 40 374 32 315 26V0H524Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.77,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,22.032,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 635 136 610 108 579L65 516L85 496C110 533 150 575 205 575C258 575 300 543 300 481C300 407 232 369 141 339L147 310C163 315 188 321 211 321C268 321 338 284 338 192C338 94 288 40 217 40C160 40 119 68 93 91C85 98 77 97 69 91C60 84 47 71 46 58C44 46 48 35 62 22C75 10 116 -12 162 -12C234 -12 424 62 424 224C424 297 373 359 285 376V378Z"></path></g></svg> per group). Gray microRNAs represent not detected. (c) Significant differences in expression levels of microRNAs. Red bar indicates an increase in primed cADSC-EVs, and blue bar indicates an increase in naive cADSC-EVs.</div>

Journal of Immunology Research

fig2

Figure 2

Figure 2: Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4<sup>+</sup> T Cells Isolated from Peripheral Blood Mononuclear Cells 

Figure 2 | Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells 