Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4<sup>+</sup> T Cells Isolated from Peripheral Blood Mononuclear Cells

<div>Differentiation into Th17 cells after coculture of PBMCs with cADSC-EVs. (a) Flow cytometric detection of CD4<sup>+</sup>IL-17<sup>+</sup> cells among CD3<sup>+</sup> T cells (gated in red square). Non-stim control: PBMCs that were not stimulated with PMA and ionomycin Stimulated control: PBMCs after coculture with cADSC-EVs and stimulated by PMA and ionomycin. (b) Comparison of CD4<sup>+</sup>IL-17<sup>+</sup> cells among CD3<sup>+</sup> T cells after coculture of PBMCs with various concentrations (1 or 3 <i>μ</i>g/ml) of naive or primed cADSC-EVs. The ratio of Th17 cells was not significantly different between naive and primed EVs at the same concentrations. 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Journal of Immunology Research

fig9

Figure 9

Figure 9: Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4<sup>+</sup> T Cells Isolated from Peripheral Blood Mononuclear Cells 

Figure 9 | Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells 