Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis

Figure 1

Expression of miR-221/222 is downregulated by propofol. (a) The inhibitory effects of propofol on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells were determined by real-time qPCR analysis (). (b) The inhibitory effects of propofol on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells were analyzed by ELISA (). (c) miR-221 and miR-222 expression in BV2 cells upon treatment with different concentrations of LPS were analyzed by real-time qPCR (); comparisons were done with 0 ng/ml LPS. (d) miR-221 and miR-222 expression in LPS-primed BV2 cells upon treatment with different concentrations of propofol were analyzed by real-time qPCR (). , , and .

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