Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis

Figure 2

Effect of miR-221/222 on activation of BV2 microglia. (a) The transfection efficiency of miR-221 and miR-222 mimics was validated by qRT-PCR analysis. (b) The effects of miR-221 and miR-222 mimics on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in BV2 cells were determined by real-time qPCR analysis (). (c) The levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from BV2 cells upon treatment with miR-221 and miR-222 mimics were analyzed by ELISA (). , , and ; comparisons were done with ctrl mimic.

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