Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis

Figure 3

Inhibition of miR-221/222 blocks LPS-mediated BV2 activation. (a) The transfection efficiency of miR-221 and miR-222 inhibitors was validated by qRT-PCR analysis. (b) The effects of miR-221 and miR-222 inhibitors on the mRNA expression of inflammatory genes (Il1b, IL6, Tnf, Ptgs2, and Nos2) in LPS-primed BV2 cells were determined by real-time qPCR analysis (). (c) The effects of miR-221 and miR-222 inhibitors on the level of inflammatory cytokines (IL-1β, IL-6, and TNF-α) in conditioned medium from LPS-primed BV2 cells were analyzed by ELISA (). , , and .

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